locan.data.transform.bunwarpj.bunwarp#

locan.data.transform.bunwarpj.bunwarp(locdata, matrix_path, pixel_size, flip=False)[source]#

Transform coordinates by applying a B-spline transformation as represented by a raw transformation matrix from BunwarpJ.

Parameters:

  • locdata (LocData) – specifying the localization data on which to perform the manipulation.

  • matrix_path (str | PathLike[Any]) – Path to file with a raw matrix from BunwarpJ.

  • pixel_size (tuple[float, float]) – Pixel sizes used to determine transition matrix in ImageJ

  • flip (bool) – Flip locdata along x-axis before transformation

Returns:

New localization data with transformed coordinates.

Return type:

LocData