locan.analysis.uncertainty

Compute localization uncertainty.

A theoretical estimate for localization uncertainty is given by the Cramer-Rao-lower-bound for the localization precision. The localization precision depends on a number of experimental factors including camera and photophysical characteristics [1] [2]. We provide functions to compute localization uncertainty from available localization properties as predicted by the Cramer-Rao-lower-bound for the localization precision.

References

[1]

K.I. Mortensen, L. S. Churchman, J. A. Spudich, H. Flyvbjerg, Nat. Methods 7 (2010): 377–384.

[2]

Rieger B., Stallinga S., The lateral and axial localization uncertainty in super-resolution light microscopy. Chemphyschem 17;15(4), 2014:664-70. doi: 10.1002/cphc.201300711

Classes

LocalizationUncertainty([meta, model])

Compute the Cramer Rao lower bound for localization uncertainty for each localization's spatial coordinate in locdata.

Functions

localization_precision_model_1(intensity)	Localization precision as function of intensity (I) according to:
localization_precision_model_2(intensity, ...)	Localization precision as function of intensity (I), PSF width (sigma_PSF) according to:
localization_precision_model_3(intensity, ...)	Localization precision as function of intensity (I), PSF size (sigma_{PSF}), pixel size (a), background (b) according to: